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Description
bsa fatty acid free cho vero mdckPremium fr CHO, Vero, MDCK & sensitive Assays SeamlessBio Bovine Serum Albumin Fatty Acid Free ist speziell aufgereinigt, um gebundene Fettsuren zu entfernen, die in Standard BSA natrlich vorkommen. Mit 98% Reinheit und <5 EU mg Endotoxin ist es die erste Wahl fr Fettsure sensitive Zelllinien (CHO, Vero, MDCK) und Lipid Metabolismus Studien. Fatty Acid Free >99% FA Entfernung durch Charcoal Delipidation Reinheit 98% Hher als Standard BSA Low Endotoxin
Premium für CHO, Vero, MDCK & sensitive Assays
SeamlessBio Bovine Serum Albumin Fatty Acid Free ist speziell aufgereinigt, um gebundene Fettsäuren zu entfernen, die in Standard-BSA natürlich vorkommen. Mit ≥98% Reinheit und <5 EU/mg Endotoxin ist es die erste Wahl für Fettsäure-sensitive Zelllinien (CHO, Vero, MDCK) und Lipid-Metabolismus-Studien.
✓ Fatty Acid Free – >99% FA-Entfernung durch Charcoal-Delipidation
✓ Reinheit ≥98% – Höher als Standard-BSA
✓ Low Endotoxin <5 EU/mg – LAL-tested
✓ Keine Interferenz – Ideal für Lipid-sensitive Assays
✓ AU/NZ/US Origin – BSE/TSE-frei
✓ Reinheit ≥98% – Höher als Standard-BSA
✓ Low Endotoxin <5 EU/mg – LAL-tested
✓ Keine Interferenz – Ideal für Lipid-sensitive Assays
✓ AU/NZ/US Origin – BSE/TSE-frei
Warum Fatty Acid Free BSA?
Standard-BSA enthält natürlich gebundene Fettsäuren (~0.5-2 mol FA pro mol BSA), hauptsächlich:
- Palmitate (C16:0) – Gesättigte Fettsäure
- Stearate (C18:0) – Gesättigte Fettsäure
- Oleate (C18:1) – Einfach ungesättigte Fettsäure
- Linoleate (C18:2) – Mehrfach ungesättigte Fettsäure
Probleme in bestimmten Anwendungen:
- Zellkultur: CHO, Vero, MDCK sind sensitiv gegenüber exogenen Fettsäuren → veränderte Wachstumsraten, beeinträchtigte Produktqualität
- Lipid-Metabolismus-Studien: Native FA interferieren mit Messungen von Lipid-Transportern, Beta-Oxidation, Lipoprotein-Metabolismus
- Hormon-Assays: FA beeinflussen Steroid-Bindung (Testosteron, Estrogen, Cortisol)
- Drug-Binding-Studies: FA konkurrieren um Bindungsstellen am Albumin
Lösung: Fatty Acid Free BSA eliminiert diese Probleme durch Charcoal-Delipidation (Aktivkohle-Behandlung) → >99% FA-Entfernung.
Technische Spezifikationen
| Parameter | Spezifikation | Test-Methode |
|---|---|---|
| Quelle | Rinderplasma (AU/NZ/US) | COO-Zertifikat |
| Reinheit | ≥98% | Elektrophorese |
| Fettsäure-Gehalt | <0.01 mol FA/mol BSA | GC-MS (>99% Entfernung) |
| Endotoxin | <5 EU/mg | LAL-Test |
| Albumin-Gehalt | ≥98 g/L | Bradford/BCA |
| Monomere | ≥95% | SEC-HPLC |
| pH-Wert | 6.8-7.5 | pH-Meter |
| Format | Lyophilized Powder | — |
| Lagerung | Raumtemperatur (versiegelt) | — |
| Haltbarkeit | 3 Jahre | — |
Standard vs. Fatty Acid Free BSA
| Eigenschaft | Standard BSA | Fatty Acid Free BSA |
|---|---|---|
| Fettsäure-Gehalt | 0.5-2 mol FA/mol BSA | <0.01 mol FA/mol BSA |
| Reinheit | ≥96% | ≥98% |
| Endotoxin | <10 EU/mg | <5 EU/mg |
| Ideal für | Western Blot, ELISA, Protein-Standard | CHO/Vero/MDCK, Lipid-Studien |
Anwendungen
1. CHO/Vero/MDCK Zellkultur
Warum diese Zelllinien Fatty Acid Free BSA brauchen:
CHO (Chinese Hamster Ovary):
- Biomanufacturing von monoklonalen Antikörpern (mAbs) und rekombinanten Proteinen
- Exogene Fettsäuren stören Glykosylierung und Produktqualität
- FDA/EMA bevorzugen FA-freie Supplements für GMP-Produktion
- Typische Konzentration: 0.5-2 g/L in serum-freien Medien
Vero (African Green Monkey Kidney):
- Vaccine Production (Polio, Rabies, Rotavirus, Dengue)
- WHO-zertifizierte Zelllinie für humane Impfstoffe
- FA-freie Bedingungen sind essentiell für regulatorische Compliance
- Verbesserte Virus-Titer bei FA-freiem BSA
MDCK (Madin-Darby Canine Kidney):
- Influenza-Vaccine Production (saisonale und pandemische Impfstoffe)
- FDA bevorzugt FA-freie Supplements
- Höhere HA (Hemagglutinin) Ausbeute
2. Lipid-Metabolismus & Fatty Acid Uptake Studies
Studien zu Lipid-Transportern:
- CD36 (Fatty Acid Translocase): Uptake-Assays ohne Background durch native FA
- FATP (Fatty Acid Transport Protein): Spezifische FA-Aufnahme-Studien
- FABPpm (Plasma Membrane Fatty Acid Binding Protein): Bindungs-Studien
Beta-Oxidation-Assays:
- Messung der mitochondrialen FA-Oxidation
- Keine Interferenz durch BSA-gebundene FA
- Verwendung radioaktiv-markierter FA (z.B. 14C-Palmitat)
Lipoprotein-Metabolismus:
- HDL/LDL-Studien
- Apolipoprotein-Bindung
- CETP (Cholesteryl Ester Transfer Protein) Aktivität
Adipozyten-Differenzierung:
- 3T3-L1 Präadipozyten → Adipozyten
- Kontrollierte FA-Zugabe für Lipid-Akkumulation
- Oil Red O Färbung ohne Background
3. Hormone-Binding Studies
Thyroid Hormone (T3, T4) Binding:
- FA konkurrieren um Bindungsstellen am Albumin
- Fatty Acid Free BSA ermöglicht präzise Bindungsstudien
- Wichtig für Schilddrüsen-Diagnostik
Steroid Hormone Assays:
- Estrogen, Testosteron, Cortisol, Progesteron
- FA beeinflussen Protein-Bindung
- Wichtig für Endokrinologie-Forschung
Drug-Protein Binding Studies:
- Pharmakokinetik-Studien
- Kompetition zwischen Drug und FA um Albumin-Bindungsstellen
- Wichtig für Drug Development
4. Serum-freie Medien Formulierung
- Präzise Kontrolle über Lipid-Zusammensetzung
- Gezielte Zugabe definierter Fettsäuren (z.B. Oleic Acid, Linoleic Acid)
- Reproduzierbare Zellkultur-Bedingungen
- GMP-konforme Medien-Entwicklung
Verfügbare Volumina
| Format | Packaging | Ideal für |
|---|---|---|
| Lyophilized Powder | 10 g | Kleine Labs, Methodenentwicklung |
| Lyophilized Powder | 50 g | Routine-Forschung |
| Lyophilized Powder | 100 g | High-throughput Labs |
| Lyophilized Powder | 500 g | Bioproduktion, GMP |
Andere Volumina & Bulk-Orders: Größere Mengen (≥1 kg) sowie kundenspezifische Formate sind auf Anfrage verfügbar. Kontaktieren Sie uns für ein individuelles Angebot.
Lagerung & Handling
Lagerung:
- Raumtemperatur (15-25°C), trocken, versiegelt
- Haltbarkeit: 3 Jahre ab Herstellung
- Nach Öffnen: Bei 2-8°C lagern, innerhalb 6 Monaten verbrauchen
Auflösen von Pulver:
- Langsam in Wasser oder Puffer (PBS, TBS) geben
- Vorsichtig schwenken (nicht vortexen)
- Bei Raumtemperatur oder 37°C lösen
- pH auf 7.0-7.4 einstellen
- 0.2 µm sterilfiltrieren für Zellkultur
Wichtig: Vermeiden Sie wiederholtes Einfrieren/Auftauen, da dies zu Proteinaggregation führen kann.
Herkunft & Qualitätssicherung
AU/NZ/US – BSE/TSE-freie Regionen (OIE-zertifiziert)
Jede Charge wird geliefert mit:
- Certificate of Analysis (CoA) – Vollständige Spezifikationen
- Certificate of Origin (COO) – AU/NZ/US Herkunft dokumentiert
- Fatty Acid Analysis (GC-MS) – <0.01 mol FA/mol BSA bestätigt
- Endotoxin-Test-Zertifikat (LAL) – <5 EU/mg bestätigt
- BSE/TSE-Freiheitserklärung
- Safety Data Sheet (SDS)
Warum SeamlessBio für BSA Fatty Acid Free?
- >99% FA-Entfernung – Charcoal-Delipidation validiert
- Reinheit ≥98% – Höher als Standard-BSA
- Low Endotoxin <5 EU/mg – Ideal für Zellkultur
- BSE/TSE-frei – AU/NZ/US Origin
- Schnelle EU-Lieferung – Lager in Deutschland, 1-3 Tage
- Kostenfreie Muster – 10g Test-Volume für qualifizierte Kunden
- Technischer Support – Bei Fragen zu Protokollen
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 11 reviews
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Product Reviews
★★★★★ 4
Baby bumps and bodyguards
Format: Kindle
Dark, emotional, and unexpectedly tender, Not Ready is an omegaverse romance that delivers found family feels, fierce protectiveness, and a very pregnant heroine who refuses to break. Vale’s on the run from a stalker, but lands in the arms of three private security alphas, cue the swoony tension, fake marriage twist, and slow-burn heat.
It’s a little gritty, a little soft, and a whole lot addictive. If you love protective alphas, high stakes, and heroines with quiet strength, this one’s a must-read.
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Reviewed in the United States on December 18, 2025
★★★★★ 5
Strong Omegaverse Comfort and a Attention Grabbing Plot
Format: Kindle
Jillian West never misses when it comes to Omegaverse, and Not Ready is no exception. This story was the perfect blend of cozy comfort and emotional depth while still delivering a strong plot.
Vale is such a powerful heroine, she is strong, capable, and determined but I love that she still allows her pack to love and take care of her. It’s that balance of independence and vulnerability that makes her so relatable.
The relationship dynamics were amazing: Bishop is steadfast and completely head over heels, Mercy is skeptical but protective in his own way, and Holt is the hesitant one whose slow fall is so satisfying to watch unfold.
The romance hits that sweet spot between insta-love and cautious build, keeping me hooked the entire way through. And that ending. Oh my god, the cliffhanger! I need the next book in this duet immediately.
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Reviewed in the United States on August 28, 2025
★★★★★ 5
Interesting
Format: Kindle
So I will say I enjoyed the story, for sure had its moments where it dragged but it was a great story. I really liked that omegas picked their alphas/make the pack. Normally the Alphas make it and the omega fits in with them which is great but I enjoyed this new version where all the power basically went to the omega. It was a nice change of pace. I can admit some of the weird bedroom stuff with her being pregnant was odd, it’s really not hard to do stuff when pregnant (I know I’ve had two and it’s normal and even encouraged at the end especially if you want the baby out). But I like the story as a whole and will read the second, I do hope the next one isn’t dragged bc it stopped being action or tense after she met her alphas and I don’t think it was brought up or properly done when they tried to do it. More sweet after she left.
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Reviewed in the United States on November 11, 2024
★★★★★ 3
I’m a little disappointed.
Format: Kindle
I usually like Jillian West’s books but this one was missing a lot for me. The pregnancy didn’t come across as real. She’s on her feet for 12 hour days but is perfectly healthy at 8 months pregnant? Yet the week she moves in all of a sudden she’s not? She is planning on actually running during one of the plot buildups. But at 8 months pregnant that’s incredibly hard to do. The lack of breathing ability and lung space, the change in body center, mass, and gravity. All of it prohibits running, unless you’re an athlete this didn’t come off as at all realistic.
I didn’t feel any connection with the alphas. There wasn’t any emotional connection. It could be because of the tense it was written in. But I didn’t get any deep feelings out of this. It came across as checking off boxes. Even the spicy scenes weren’t really believable for me. I wanted to see them fall for her, and it just kind of all fizzled. Even Bishop.
One thing I did really like was the ending. I did not see it coming and I’m interested in reading book two because of it. But on the whole this book was mostly disappointing for me.
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Reviewed in the United States on March 16, 2024
★★★★★ 4
4.25 stars
Format: Kindle
Vale is an 8 month pregnant omega working as a waitress at a strip club and a cam girl. She starts to get very creepy vibes from a regular at the club, and her baby daddy ghosted her. She has had an online relationship with a man named Bishop through her cam girl status. One night, bishop was paying to watch her sleep and ansthe creepy regular Andrew break in and watch her sleep he tells vale to come to him at his business now. She flees and finds herself at a large security company with some.hot of alphas who are there to help her. This imegaverse is a little different than I have read, but I am thoroughly enjoying it. Vale is not a traditional omega she was raised by a single beta mom, and the alphas are not normal alphas they have never really loved pack life. But they are ruthless mercenaries. They need her, and she needs them. I love the aspect of the stalker and now the plot twists at the end, so so good. Sometimes, it seemed a little slow and stale mated, but since this a duet, I think It was just her starting to have Vale get to know her alpha suitors. Cliffhanger for sure with this one.
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Reviewed in the United States on September 9, 2024