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Description
Rat WNT4 (Protein Wnt-4) ELISA KitThe Rat (WNT4) Protein Wnt 4 ELISA Kit measures Protein Wnt 4 in Rat samples. The plate has been pre coated with Rat WNT4 antibody. WNT4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat WNT4 Antibody is added and binds to WNT4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated WNT4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
The Rat (WNT4) Protein Wnt-4 ELISA Kit measures Protein Wnt-4 in Rat samples. The plate has been pre-coated with Rat WNT4 antibody. WNT4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat WNT4 Antibody is added and binds to WNT4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat WNT4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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