SKU: 24136223648

Rat GNRH (Gonadotropin-Releasing Hormone) ELISA Kit

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Description

Rat GNRH (Gonadotropin-Releasing Hormone) ELISA KitThe Rat GNRH (Gonadotropin Releasing Hormone) ELISA Kit measures GnRH in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with GnRH. GnRH in the sample or standard competes with a fixed amount of biotin labeled GnRH for sites on a pre coated antibody specific to GnRH. Free components are washed off.

The Rat GNRH (Gonadotropin-Releasing Hormone) ELISA Kit measures GnRH in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with GnRH. GnRH in the sample or standard competes with a fixed amount of biotin-labeled GnRH for sites on a pre-coated antibody specific to GnRH. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of GnRH in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 24136223648

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4.5 ★★★★★
Based on 22 reviews
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Verified Purchase
Monica
Phoenix, US
★★★★★ 5
Good product
Color: Life's A Paw-Ty, Size: 4.75" H X 5.25" W X 2" D
A favorite squeky toy
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 18, 2025
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Verified Purchase
Stacy
Phoenix, US
★★★★★ 5
My Dog's All-Time Favorite Toy
Color: Pink, Style: Grunt Sound
The first one of these I ever got her was at Petco on sale for $1 - best $1 ever! The reviews are right, though. The toy doesn't last forever and it's not good for aggressive chewers. In my opinion, however, that doesn't make it a bad toy. Here's why: Aggressive chewers comments: Getting my sister's French Bulldog a stuffed toy (that's shredded in 5 minutes), compared to my Pitbull who does not tear up any toys, but is a stronger chewer when it comes to bones. My pup does not tear this up at all, but my sister's dog would. My point is: Don't get your dog a soft toy if your dog ruins soft toys. Regarding the not long-lasting comments: the honking noise mechanism inside eventually pops inside the hole, rendering it honkless. Ours lasted about 6 months. It's pretty much impossible to fix unless you want to trouble yourself to fix it for 1 good honk each fix. I would, but I do also have a job to get to. My dog does still carry it around in it's noiseless state, but she doesn't play with it with the same enthusiasm. How did I resolve this? I bought her 5 😂 She's only on her second, but I decided to stock it because they were on sale for $5.10 - I don't think I will get rid of any of them. I will just let her play with them on rotation until they fall apart. Besides all that, my dog just simply loves this toy. I have never seen her so happy & playful with a toy. I can't promise this for your dog, as I can only tell you about mine. We even have a "Sheepy" song. [I have not been paid or given free Sheeps - my Sweet Pea just really loves her Sheepy]
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Reviewed in the United States on March 26, 2024
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Verified Purchase
Whidbey Mary
Omaha, US
★★★★★ 5
Loud! Just Like She Likes It
Color: Hedgehog, Style: Big Squeak Hedgehog
My Great Dane loves this noisy toy. She prefers squeaky toys with loud, unusual squeakers. (I need to invest in earplugs.) She's not an agressuve chewer or rough player so this should last a long time. Like most squeaky toys she has, the squeaker wears out before the physical toy does and once the noise is gone she won't play with it any more. If others have a similar issue...check with your local animal shelter. Some (not all) will disinfect used toys for the dogs in their care.
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Reviewed in the United States on November 1, 2025
S
Verified Purchase
Sport
Lowell, US
★★★★★ 5
Good toy
Color: Gator, Style: Accordionz (Grunt Sound)
Fun toy, holding up really well.
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Reviewed in the United States on June 1, 2026
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Verified Purchase
Reviewer
Louisville, US
★★★★★ 1
Fun while it lasts…
Color: Pink, Style: Grunt Sound
Fun while it lasts. Doesn’t last very long. This is our third one and last maybe a few weeks. The part that makes noise comes detached inside which causes to toy to no longer make noise, but also can be dangerous if it comes out.
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Reviewed in the United States on June 11, 2026

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