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Description
Rat MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B) ELISA KitThe Rat MAP1LC3B (Microtubule associated proteins 1A 1B light chain 3B) ELISA Kit measures MAP1LC3B in Rat Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MAP1LC3B antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
The Rat MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit measures MAP1LC3B in Rat Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MAP1LC3B antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with MAP1LC3B bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of MAP1LC3B in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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