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Description
Human TSP-1 (Thrombospondin 1) ELISA KitThe Human (TSP 1) Thrombospondin 1 ELISA Kit measures Thrombospondin 1 in samples. The plate has been pre coated with Human THBS1 antibody. THBS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human THBS1 Antibody is added and binds to THBS1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated THBS1 antibody. After incubation unbound Streptavidin HRP is washed away during a
The Human (TSP-1) Thrombospondin 1 ELISA Kit measures Thrombospondin 1 in samples. The plate has been pre-coated with Human THBS1 antibody. THBS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human THBS1 Antibody is added and binds to THBS1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated THBS1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human THBS1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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