Monomeric CRP ELISA

Monomeric CRP ELISAThe monomeric CRP (mCRP) ELISA is an enzyme immunoassay for the quantitative determination of monomeric CRP (mCRP) in human serum and plasma. Human Monomeric ELISA specifically determines monomeric CRP (mCRP) without any reactivity with pentameric CRP (pCRP). The microtiter plate is coated with the antibody specifically binding the mCRP. The human serum and plasma are incubated in the plate with the capture antibody. The specimen is washed out and the

The monomeric CRP (mCRP) ELISA is an enzyme immunoassay for the quantitative determination of monomeric CRP (mCRP) in human serum and plasma.

Human Monomeric ELISA specifically determines monomeric CRP (mCRP) without any reactivity with pentameric CRP (pCRP).

The microtiter plate is coated with the antibody specifically binding the mCRP. The human serum and plasma are incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue color. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of mCRP in the specimen. The concentration of mCRP in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.