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Description
Human AD7c-NTP (neuronal thread protein) ELISA KitThe Human AD7c NTP (neuronal thread protein) ELISA Kit measures AD7c NTP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti AD7c NTP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
The Human AD7c-NTP (neuronal thread protein) ELISA Kit measures AD7c-NTP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti AD7c-NTP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with AD7c-NTP bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of AD7c-NTP in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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