Human anti 21-OH Ab (anti 21-hydroxylase antibody) ELISA Kit

Human anti 21-OH Ab (anti 21-hydroxylase antibody) ELISA KitThe Human anti 21 OH Ab (anti 21 hydroxylase antibody) ELISA Kit measures anti 21 OH Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human anti 21-OH Ab (anti 21-hydroxylase antibody) ELISA Kit measures anti 21-OH-Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antigen. Then, it binds with anti 21-OH Ab bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti 21-OH Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.