SKU: 80586777420

Mouse TNFSF11/RANKL (Tumor necrosis factor ligand superfamily member 11) ELISA Kit

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Description

Mouse TNFSF11/RANKL (Tumor necrosis factor ligand superfamily member 11) ELISA KitThe Mouse TNFSF11 RANKL (Tumor necrosis factor ligand superfamily member 11) ELISA Kit measures TNFSF11 RANKL in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNFSF11 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation,

The Mouse TNFSF11/RANKL (Tumor necrosis factor ligand superfamily member 11) ELISA Kit measures TNFSF11/RANKL in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNFSF11 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TNFSF11 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TNFSF11 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 80586777420

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4.4 ★★★★★
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Niall
New York, US
★★★★★ 5
Amazing shoes!
Size: 5 Wide Big Kid, Color: Black/Pink
Runs true to size .Fits wide feet and they look amazing. They're also splash proof.They also have amazing grip.Overall amazing shoes highly recommend.
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Reviewed in the United States on May 10, 2026
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Addu
Boise, US
★★★★★ 5
Great tennis shoes!!
Size: 4.5 Big Kid, Color: Black/Orange
The best shoes my son had this past year. He loves them and has worn them continuously, not only for running and training, but also for daily use. He really likes them and says they are very comfortable.
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Reviewed in the United States on February 4, 2026
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Marissa C.
Chelsea, US
★★★★★ 5
Size up
Size: 2 Big Kid, Color: Black/Pink
My daughter loves them. However they run a size small so we had to size up. She is anywhere from a 13-1 and we had to get a 2 in these. Overall great show and quality. She also just learned how to tie her shoe (7yo) and they are easy for her to tie. Style is great for running and sports too. Overall great value for the money.
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Reviewed in the United States on May 21, 2025
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MJE
San Leandro, US
★★★★★ 4
These shoes make a great first impression, but beware of the stingy toe box.
Size: 13.5 Wide Big Kid, Color: Black/Orange
These shoes make a good first impression. They look sharp and appear to be well-made for running shoes. They also get bonus points for having actual laces instead of the common velco/fake laces combo. However, my son struggled to get them on at first due to limited room in the toe box. He has wide feet as well as high arches and the shoes were very snug across the top of his foot initially. Once he got them on, we determined that they were both wide enough (which is a rarity in itself) and the right size – just tight across the top. I actually had to remove the insoles to get them on his feet at first. After a few minutes I tried again with the insoles and he wore them around the house for a few hours, at which point the upper material had relaxed and/or the footbed had started to take on the shape of his foot, in effect creating more wiggle room. By then he didn't want to take them off, so they must have gotten more comfortable after that brief break-in period. If they were still too tight I'm sure he would have complained. I'll update the review if the shoes wear out prematurely or there is anything else noteworthy to report.
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Reviewed in the United States on August 15, 2024
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Grantham, US
★★★★★ 5
Awesome shoes
Size: 6 Wide Big Kid, Color: Black/Orange
Awesome shoes, great tread, comfortable!
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Reviewed in the United States on February 25, 2026

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