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Description
Human LIMK1 (Lim Domain Kinase 1) ELISA KitThe Human (LIMK1) Lim Domain Kinase 1 ELISA Kit measures Lim Domain Kinase 1 in samples. The plate has been pre coated with Human LIMK1 antibody. LIMK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LIMK1 Antibody is added and binds to LIMK1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LIMK1 antibody. After incubation unbound Streptavidin HRP is washed away during
The Human (LIMK1) Lim Domain Kinase 1 ELISA Kit measures Lim Domain Kinase 1 in samples. The plate has been pre-coated with Human LIMK1 antibody. LIMK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LIMK1 Antibody is added and binds to LIMK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LIMK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LIMK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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