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Description
Rat Na-K-ATPase ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Na-K-ATPase (Na-K-ATPase) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Na-K-ATPase (Na-K-ATPase) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Na-K-ATPase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Na-K-ATPase (Na-K-ATPase) is an enzyme present in the cell membranes of all animal cells (an electrically charged transmembrane ATPase). It plays several roles in cellular physiology. The Na⁺/K⁺-ATPase enzyme is active (i.e., it uses energy from ATP). For every ATP molecule used by the pump, three sodium ions are exported and two potassium ions are imported, resulting in a net output of positive charge per pump cycle. This enzyme belongs to the P-type ATPase family. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.2 ★★★★★
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Product Reviews
★★★★★ 5
The BEST sunscreen I've found
Style: SPF 50, Size: 3 Fl Oz (Pack of 1)
This is my MUST HAVE sunscreen. I've had almost every type of skin cancer (thankfully, caught early), so I NEED good sun protection, especially on my face. I have very fair skin. This product leaves a bit of a white cast, but after a short period of time, that white case is absorbed. The white cast of a mineral sunscreen might look chalky on a person with darker skin. The BEST thing about this sunscreen is I can put it on my eyelids and under my eyes, it doesn't irritate my eyes AT ALL. The other well-known sunscreen I used for a long time really made my eyes sting if a bit got into them. This is my favorite sunscreen of all time.
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Reviewed in the United States on February 3, 2025
★★★★★ 4
Solid mineral SPF with light coverage effect
Style: SPF 50, Size: 3 Fl Oz (Pack of 1)
I’m used to La Roche-Posay’s chemical sunscreens, so this was my first time trying their mineral version. The texture is actually quite nice for a mineral formula. It spreads evenly and doesn’t feel gritty or chalky. That said, it does take a bit longer to fully absorb compared to chemical SPFs.
I’m already very pale, and I still notice a slight white cast. It’s not extreme, but it’s there. To be fair, most mineral sunscreens tend to do this, especially those using zinc oxide and titanium dioxide.
Interestingly, on my skin it almost behaves like a very light tinted sunscreen. It slightly blurs and softens the appearance of minor blemishes and redness, which I appreciate on minimal-makeup days.
It provides broad-spectrum SPF 50 protection and feels gentle on sensitive skin. I haven’t experienced irritation, pilling, or breakouts. It layers reasonably well over moisturizer but benefits from being applied in thin, well-blended layers, which adds some time to my morning routine so it's a little inconvenient.
Overall, it’s a solid mineral option with good protection. Just expect a bit more blending time and a subtle cast.
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Reviewed in the United States on February 24, 2026
★★★★★ 5
Great for pregnancy (or regular use)
Style: SPF 50, Size: 4.05 Fl Oz (Pack of 1)
I searched far and wide for a safe mineral-based sunscreen for pregnancy. I settled on this one and I was not disappointed. It saved my skin from a lot of sunburn this summer!
As other reviewers mentioned, it is harder to rub into the skin and can leave a whiteish tint, but that’s a small price to pay for a pregnancy-safe option.
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Reviewed in the United States on September 22, 2025
★★★★★ 1
Terrible, look like a ghost
Style: SPF 50, Size: 3 Fl Oz (Pack of 1)
I love La Roche-Posay but unfortunately, I finally came across the first product I truly hate from them. This product is a complete white mask on your face and does not settle in at all. You’ll be walking around looking like a ghost all day and it gave me a mild irritation to my eyes.
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Reviewed in the United States on May 29, 2026
★★★★★ 3
Leaves white cast, works okay under makeup.
Style: SPF 50, Size: 3 Fl Oz (Pack of 1)
First time using this sunscreen. I have very sensitive skin and thought since my skin tolerates their cleansers and moisturizers surely it should tolerate the sunscreen. So far so good. It’s very calming on my skin (maybe because of the zinc), but it leaves a noticeable white cast especially if you have dark skin. I wear mine under makeup and have a separate sunscreen from another brand on days where I don’t want to wear makeup. The white cast actually acts as a blank base and helps my foundation to lay well. A tip I learned from makeup artists years ago. Sunscreens that leave a white cast cancels out your natural color and acts as a corrector, concealer and foundation to lay better and look more like skin. Think of it like painting on a blank canvas. The texture is not lotion like and is actually quite thick. make sure to moisturize well before apply this sunscreen. I’ll write an update if I notice any negative issues, but so far I’m content with it because of the purpose I'm using it for.
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Reviewed in the United States on October 23, 2023