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Description
Mouse IL-6R ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 ng/mL). Then dilute to the following concentrations: 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 200ng/mL standard working solution into the first EP tube and mix thoroughly to make a 100ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 6 Receptor (IL-6R) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Interleukin 6 Receptor (IL-6R) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 6 Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The interleukin-6 receptor (IL6R), also known as CD126 (cluster of differentiation 126), is a type I cytokine receptor. It is a protein complex composed of the IL-6 receptor subunit (IL6R) and the interleukin-6 signal transducer glycoprotein 130. It regulates cell growth and differentiation and plays a vital role in immune responses. Dysregulation of IL6 and this receptor secretion is implicated in the pathogenesis of numerous diseases, including multiple myeloma, autoimmune diseases, and prostate cancer. In melanocytes, IL6R gene expression may be regulated by MITF. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.2 ★★★★★
Based on 807 reviews
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Product Reviews
★★★★★ 4
Amazon should know how to deliver books without damaging.
Format: Board book, Format: Board book
This is a fantastic book and the back has fun facts, plus a dedication the minor planet Pluto that was not included. If you are Gen-x you know that acknowledging Pluto on the back just warms yor heart.
Most of the science info is on the back cover. The board book focuses on naming the planets and the artistry. I think this is great and the extra facts on the back are better for older kids who might want to pretend they are too big for baby books.
This is a really beautiful book, but there was damage. The lip is all suffered up and marred (2nd picture) and where you open the book (1st picture) there is already a wear crease. I blame the defects on Amazon, but a cellophane wrap might have helped prevent the damage
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 2, 2025
★★★★★ 5
Fun way to learn about the planets
Format: Board book
An awesome book. One of my toddlers favorites. Love the cut outs and pictures as well as the content. Its a fun way to learn about the planets
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 15, 2026
★★★★★ 5
Such a great and important book
Format: Kindle
"Perseverance Makes Me Stronger" by Elizabeth Cole is an exceptional children's book that beautifully captures the essence of determination, resilience, and personal growth. Through the endearing story of young Nick and his roller skating journey, Cole delivers a powerful message that will inspire and motivate young readers.
The book revolves around Nick, a little boy who receives a pair of shiny new roller skates. Excitement quickly turns to frustration when Nick realizes that learning to skate is not as easy as he imagined. Discouraged by the challenges he faces, Nick contemplates giving up. However, with the unwavering support and guidance of his father, he learns the true meaning of perseverance.
Cole's storytelling is enchanting, and the relatable nature of Nick's struggles immediately captures the attention of young readers. The writing style is simple and accessible, making it easy for children to understand and engage with the narrative. The book is filled with colorful illustrations that vividly depict Nick's roller skating journey, further enhancing the reading experience.
One of the most commendable aspects of "Perseverance Makes Me Stronger" is the valuable life lesson it imparts. Through Nick's story, children learn the importance of persistence and the rewards that come with not giving up. The book teaches young readers that setbacks and challenges are a natural part of learning and growth, and that by persevering, they can overcome any obstacle.
The character of Nick is endearing and relatable, and children will easily connect with his feelings of frustration and self-doubt. As the story unfolds, they will witness his transformation, gaining a sense of empowerment and encouragement. Nick's journey serves as an inspiring example for young readers, showing them that with determination and support, they too can achieve their goals.
"Perseverance Makes Me Stronger" is an excellent tool for parents, educators, and caregivers to introduce children to the concept of perseverance in an engaging and relatable way. It opens up conversations about the importance of persistence, resilience, and the value of learning from mistakes. The book provides an opportunity for children to develop a growth mindset and understand that their efforts and dedication can lead to success.
In conclusion, "Perseverance Makes Me Stronger" by Elizabeth Cole is a heartwarming and impactful children's book that celebrates the power of perseverance. Through Nick's roller skating adventure, young readers are encouraged to embrace challenges, never give up, and discover their own inner strength. This delightful tale is sure to captivate young minds and leave a lasting impression, making it a valuable addition to any child's library.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 8, 2023
★★★★★ 5
Excellent children’s book on perseverance!
Format: Kindle, Format: Kindle
This is the seventh story about little Nick in the “World of Kids’ Emotions” series by author Elizabeth Cole.
I have read all the books in this series and have shared them with my young grandson over the years. Emotions can be difficult for boys and girls too!
I like how in this story Nick is trying rollerskating! He’s not doing very well and gets discouraged. Fortunately, his father offers some encouragement to persevere and keep at it!
His mother tried baking cakes. At first she did not do a good job, but later mastered the skill of baking cakes. Other examples are used throughout the book to show that trying to do your best and having a goal are great ways to approach persevering!
“Perseverance makes me stronger.” I love the positive messages that are in this book. Positive self talk and encouraging oneself are helpful tools. “No matter the outcome, at least I did try!” ”My dreams will come true because I believe in myself.”
There are some positive affirmations at the end of the book. And also a free coloring page that can be downloaded with a scan code.
The illustrations are very nice to look at and colorful. The artist did a lovely job. The illustrations go along with the storyline nicely.
The writing in this book has a pleasant flow with lots of rhyming words. “Perseverance means not quitting right away, but keep trying more and more, day after day.”
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Reviewed in the United States on June 11, 2023
★★★★★ 5
Great book to never give up
Format: Paperback
Great book! My son loves it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 13, 2026
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