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Description
Human BACH1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend them in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, the PBS volume can be reduced appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cerebrospinal Fluid Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against transcription regulator protein BACH1 (BACH1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of transcription regulator protein BACH1 (BACH1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transcription regulator protein BACH1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The transcriptional regulator BACH1 is a protein encoded by the BACH1 gene. This gene encodes a cap-n-collar type transcription factor belonging to the basic region leucine zipper family (CNC-bZip). The encoded protein contains extensive complex, triad, triad/poison, and zinc finger (BTB/POZ) domains, which are atypical among CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and the formation of homo- and/or hetero-multimers. The C-terminus of this protein contains a leucine zipper from the bzip_maf family. When heterodimerized with MafK, this protein functions to inhibit Maf recognition elements (MAREs), resulting in transcriptional repression. Multiple alternatively spliced transcript variants have been identified for this gene. Pathways implicated in this gene include the comprehensive breast cancer pathway and the BRCA1 pathway. Gene ontology annotations associated with this gene include DNA-binding transcription factor activity and heme binding. An important homologue of this gene is BACH2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cerebrospinal fluid, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
Based on 222 reviews
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Product Reviews
★★★★★ 5
I’m happy with it
Size: L50 Hybrid-renewed, Size: L50 Hybrid-renewed
Works great.
Edit:
After a week that I wrote works great, it had the issue that when it starts cleaning it looses power and shuts off after it leave the dock. When it disengages from charging it’s dead basically so unfortunately I returned it. But I will buy it again I was happy with it.
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Reviewed in the United States on March 11, 2025
★★★★★ 1
Charging base makes awful high pitch noise.
Size: L50 Hybrid-renewed
It cleans fine but the charging base emits a terrible high pitch noise. I have to unplug it when nearby. Cats no longer want to sleep in the same room as the vacuum base. Update: A little over a year later and it appears the vacuum's onboard wifi module has passed the rainbow bridge to electric heaven. Can no longer connect the L50 to the app or local wifi. Tried resetting, reverting to older version of app, changing my wifi to 2ghz only. Trying setup in airplane mode. Nothing works.
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Reviewed in the United States on September 25, 2024
★★★★★ 5
It worked!
Set name: 10 Jumbo, Set name: 10 Jumbo
I bought this to fit a full size 4" memory foam mattress topper. I gotta say it took 3 of us. 1 to lay on the memory foam and 2 of us to get it shoved in and keep pulling the bag up. But it actually fit! My arm was dying using the hand pump so we used a vacuum. It worked better than the hand pump. Due to what I was storing it did not shrink very flat, but compared to the size of the memory foam it shrunk a lot and will definitely save space!
The bags arrived today so I dont know how well it holds up long term but the bag was very sturdy yanking it up around the foam. Didn't tear or stretch. And it actually fit!
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Air comes out and STAYS out!
Set name: 10 Medium
I’ve tried so many different versions of these vacuum storage bags over the years, and this company seems to have finally figured it out. They’re absolutely not exaggerating about the 80% space savings. What really impressed me is the pump design — it actually screws onto the bag, so it stays securely attached while removing the air instead of constantly slipping off. It also has a stop valve built in, which prevents air from escaping when you remove the pump to put the cap back on. Such a smart design improvement over other brands I’ve used.
I bought the medium 10-pack to store my winter clothes in two 66-quart storage bins, and they fit surprisingly well. That said, part of me wishes I had also picked up a few of the smaller sizes just because they’d probably make stacking and organizing even easier. Overall though, I’m really impressed with the quality and design.
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Reviewed in the United States on May 20, 2026
★★★★★ 5
Great bags!
Set name: 10 Jumbo
These space bags are awesome! The company gives you a hand pump but your vacuum works great. They also give you tabs to slide over the ziplock part which works great. I bought the jumbo bags and I can’t believe what I was able to fit in them. More than one summer king size comforter in a bag, and 1 oversized extra large and thick king size winter comforter with shams and sheets in one bag. Other bags had several queen size quilts or comforters and decorative pillows. I highly recommend these bags. The price is great too.
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Reviewed in the United States on May 25, 2026